Maintenance of genome integrity is essential for the proper functioning and survival of all organisms. Accumulating evidence suggests that chromosomal rearrangements found in cancers result from conflicts between transcription and replication machineries, which can induce DNA damage. Our team is studying the causes and consequences of such "traffic accidents" on replicating DNA and how cells deal with these genotoxic events to preserve genomic stability. Our approach combines state-of-the-art methods of molecular and cell biology with advanced microscopy techniques and a broad range of biochemical methods including mass spectrometry-based proteomic profiling.
Causes of transcription-replication conflicts
Resolution of transcription-replication conflicts